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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has become observed in many tumors, which results in chromosome instability, and thus might contribute to tumorigenesis. This protein was found to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are actually explained. Two more splice variants have been reported but in-depth nucleotide sequence details is not still obtainable.

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Blocking peptides are peptides that bind exclusively into the focus on antibody and block antibody binding. These peptide commonly incorporates the epitope regarded because of the antibody.

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This information outlines further more doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...

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This manual outlines additional attainable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...

Antibodies sure to the blocking peptide no more bind towards the epitope over the goal protein. By evaluating the staining in the blocked antibody versus the antibody on your own, you can see which staining is unique.

Goal Data Cyclin E belongs into the extremely conserved cyclin spouse and children, whose users are characterized by a remarkable periodicity in protein abundance from the mobile cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic occasion. Cyclin E types a complex with and functions as a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and is also degraded as cells progress by S period.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation check here by the two antibodies...

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